Transfer Buffer

T-Pro Transfer Blotting Buffer (10X),

T-Pro Semi Dry Transfer Blotting Buffer (10X)


Transfer of proteins from the gel onto the membrane is achieved in three ways: simple diffusion, vacuum-assisted solvent flow, and electrophoretic elution. Electrophoretic elution is the method of choice because of its ease, speed and completeness of transfer. Electrophoretic elution can be achieved either by wet or by semi-dry transfer. T-Pro provides following buffers that are useful to perform the electrophoretic elution in western blotting.