T-Pro Biotechnology


for your life science lab

Gel Casting Buffer

T-Pro Separating or Resolving Buffer (4X)

T-Pro Stacking Buffer (4X)

 

T-Pro Separating or Resolving Buffer (4X)

Method

For 100ml of gel solution (Cast GEL), add 25ml of Separating or Resolving buffer and different volume of acrylamide/bis solution, 10% ammonium persulfate, TEMED and ddH2O as indicated in table 1. Swirl gently to mix. Pour the solution into the gel casting cassette. The gel should begin to polymerize in 30 minutes。 

 

T-Pro Stacking Buffer (4X)

Method

For 25ml of gel solution (Stacking GEL), add 6.5ml of Stacking buffer and different volume of acrylamide/bis solution, 10% ammonium persulfate, TEMED and ddH2O as indicated in table 1. Swirl gently to mix. Pour the solution into the gel casting cassette. The gel should begin to polymerize in 40 minutes。