T-Pro Biotechnology

 T-Pro Total Exosome Isolation Reagent (for cell culture media) 

T-Pro Total Exosome Isolation Reagent (for serum) 

T-Pro Total Exosome Isolation Reagent (for urine)

Exosomes are small vesicles (30–120 nm) containing RNA and protein that are secreted by various types of cells in culture, and found in abundance in body fluids including blood, saliva, urine, and breast milk. Exosomes are thought to function as intercellular messengers, delivering their cargo of effector or signaling macromolecules between specific cells, however, their formation, the makeup of the cargo, and biological pathways in which they are involved remain incompletely understood. The biological study of exosome function and trafficking requires the isolation of intact exosomes, but the current methods used are tedious, non-specific, and difficult.

Protocol easy to perform

- No time consuming

- No ultracentrifugation required

- Isolate exosomes from cell culture supernatants or biofluids

- Able to isolate the overall exosome population in a sample

- Isolate exosomes from a small volume of sample (plasma/serum 100 ul)

- For complex biofluids as plasma, no thrombin pretreatment is required

- Isolated exosomes are intact and suitable for different downstream analyses

T-Pro Total Exosome Isolation reagent (from cell culture media) contains reagents sufficient for processing 200~1000 mL of cell culture media.

T-Pro Total Exosome Isolation reagent (from serum) contains reagents sufficient for processing 25-125 mL of serum.

T-Pro Total Exosome Isolation reagent (from urine) contains reagents sufficient for processing 200~1000 mL of urine.

T-Pro Total Exosomes Isolation Reagent is stable for 2°C ~ 8°C