Stain solution
T-Pro EZ Stain Protein Gel Solution
This product is supplied as a ready-to-use solution containing T-Pro EZ Stain Protein Gel Solution for gel staining after electrophoresis.
Direction for Use
After electrophoresis, wash the gel in a tray with 50 ∼ 100 mL deionized water for 5 minutes three times.
Remove the water used for washing, add 25 ∼ 50 mL T-Pro EZ Stain Protein Gel Solution into the tray, and let the gel stain for 15-30 mins while shaking gently at room temperature.
Destain the gel in 50 ∼ 100 mL deionized water for 30 mins - 1 hour.
If the background is high, destain the gel in 50 ∼ 100 mL deionized water for more than 1 hour or overnight at room temperature.
T-Pro Ponceau S Stain Solution
T-Pro Ponceau S Stain Solution is a ready‑to‑use membrane stain for evaluating protein transfer efficiency or for use in total protein normalization for western blot analysis. The stain has minimal nonspecific interactions with nitrocellulose or PVDF membranes, providing a reliable method for visualization of membrane‑bound proteins. Staining with Ponceau S Stain Solution results in pink-reddish protein bands that are easily photographed and reversed in less than 5 minutes. Subsequent immunodetection is unaffected, as the stain does not alter membrane‑bound proteins.
• Do not dilute before use. Ponceau S Stain Solution is supplied in a ready‑to‑use format.
• Ensure that membranes are completely immersed in staining solution throughout the incubation.
• Ensure that all staining solution is removed before blocking membranes for fluorescent western blot detection.
