Blocking Buffer

T-Pro Fast Blocking Buffer 

T-Pro Protein Free Blocking Buffer

T-Pro Blocking Buffer

• The usage as described in these instructions may differ from other blocking solutions.

• Use the blocking buffers at the supplied concentration; do not dilute blocking buffer.

• A final concentration of 0.05% Tween-20 Detergent in the blocking buffer often improves blocking; however, it is not required nor recommended for all systems.

• The protein-free blocking buffers may be used as a protein stabilizer for drying antigen- or antibody-coated microplates. Dry plate completely before sealing in a plastic bag with desiccant. 

The T-Pro Blocking Buffers contain a proprietary compound for blocking excess binding sites in Western or ELISA blotting. This blocking buffer reduces or eliminates many of the problems encountered with traditional protein-blocking reagents, such as cross-reactivity and interference from glycosylation. Additionally, T-Pro Blocking Buffers are compatible with antibodies and avidin/biotin systems.

* Continue the blotting procedure do not using the T-Pro Blocking Buffer to dilute primary and secondary antibodies