T-Pro Biotechnology


for your life science lab

Western Blot Stripping Buffer

T-Pro Western Blot Stripping Reagent

T-Pro Western Blot Stripping Reagent II 

T-Pro Western Blot Stripping Reagent III

Introduction:

Stripping Reagent for Western Blot is formulated to effectively remove the antibodies from Western blot that have been developed with chemiluminescence. The membrane can be nitrocellulose or PVDF/nylon. The stripped membrane can be re-probing for the Western Blot and for mass spectrometry.

Reuse of blots offers many advantages

•  Effective use of sample that is available in limited amounts.       
•  Comparison of images obtained with different antibodies in the same blot.
•  Confirmation of results with the same or different antibodies.
•  It is simple, more economical and less time consuming to reprobe and mass spectrometry

Features

-  Strip blot in 5~15 minutes.
-  Incubation at room temperature.
-  No stench smelling and without addition of 2-mercaptoethanol or its analogs.
-  A propriety formulation with an eco-friendly and non-hazardous organic solvent (with light odor) and can completely disassociate the bound antibodies

Protocol of T-Pro Western Blot Stripping Reagent I

1. Pour 10 ml stripping reagent to a clean container and put the membrane in the container. Make sure that the membrane is fully submerged with the stripping buffer.
2. Incubate the membrane in stripping reagent at room temperature for 3 minutes with strong agitation for twice. For the high affinity antibodies, the incubation time need to be optimized, 5~10 minutes stripping at 37℃ is usually sufficient for most of antibodies.
3. Wash the membrane twice by using TBS-T or PBS-T at room temperature in large volumes (e.g. 100 ml) for 3 minutes.
Stripping working time 3 minStripping Reagent store RT

 

T-Pro Western Blot Stripping Reagent II

1. Pour 10 ml stripping reagent to a clean container and put the membrane in the container. Make sure that the membrane is fully submerged with the stripping buffer.
2. Incubate the membrane in stripping reagent at room temperature for 10~15 minutes with strong agitation for twice. For the high affinity antibodies, the incubation time need to be optimized, 15~20 minutes stripping at 37℃ is usually sufficient for most of antibodies.
3. Wash the membrane twice by using TBS-T or PBS-T at room temperature in large volumes (e.g. 100 ml) for 3 minutes.
Stripping working time 10 minStripping Reagent store RT

 

T-Pro Western Blot Stripping Reagent III

1. Pour 10 ml stripping reagent to a clean container and put the membrane in the container. Make sure that the membrane is fully submerged with the stripping buffer.
2. Incubate the membrane in stripping reagent at room temperature for 30 minutes. For the high affinity antibodies, the incubation condition need to be optimized, stripping at 65℃ is usually sufficient for most of antibodies.
3. Wash the membrane three times by using TBS-T or PBS-T at room temperature in large volumes (e.g. 100 ml) for 10 minutes.
Stripping working time 30 minStripping Reagent store RT