T-Pro Biotechnology


for your life science lab

Transfection Reagent (non-liposomal)

T-Pro NTR I (non-liposomal transfection reagent I)

T-Pro NTR II (non-liposomal transfection reagent II)  

Description:

T-Pro NTR I or II Transfection Reagent is a proprietary formulation for the transfection of DNA and RNA into eukaryotic cells and forms the covalent binding with nucleic acid to provide the highly efficiency and low cytotoxicity.
T-Pro NTR I or II Transfection Reagent can be used in many cell types including HEK293, 293T, 293E, BHK, CHO, COS1, COS7, HeLa, Vero, PC3, L929, NIH 3T3, Human Foreskin Fibroblasts (HFF) primary cells, Bovine Aorta Endothelial cells (BAEC), Sf9 and Sf21 Furthermore, T-Pro NTR I or II Transfection Reagent also has high transfection efficiency when applied in other eukaryotic cells.

Properties:

-  Low cytotoxicity for most of tumor cell lines and primary cells
-  Exceptional transfection efficiency of a broad range of cell types
-  Efficient transfection with or without serum
-  High levels of recombinant protein production
-  Simple, robust transfection procedure  

 

 

 

Procedure:

Form the DNA (μg): Complex (for 24 wells plate ) of T-Pro NTR I or II Transfection Reagent (μl)
l          30 μl/well in a sterile plastic tube of Add the Serum-free Culture medium (without antibiotics).
l          Add plasmid DNA 1.0 μg/well and mix by gentle pipetting.
l          Add T-Pro NTR I or II 1.0~4.0 μl/well and mix by gentle pipetting.
l          Incubate the mixture of DNA and T-Pro NTR I or II solution at room temperature for 15 minutes.
l          Add DNA/ T-Pro NTR I or II mixture to cells in each well and mix by gentle and shaking plate. Incubate cells in CO2 incubator for 18~48 hr.