T-Pro Biotechnology


for your life science lab

Transfection Reagent (in vivo)

 

Description:
T-Pro G-Fect (in vivo) Transfection Reagent is a sterile solution of a cationic, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery in vivo. Reagent causes no detectable inflammatory response and is sutable for DNA administration via various routes, including intravenous, intraperitoneal, intratumoral injection, etc.
Reagents to be Supplied by the User:
Reagent A or Sterile 5% w/v glucose solution for dilution of DNA.
General Considerations:
DNA Quality Requirements
  DNA quality is critical for successful transfection. AN A260/A280 ratio of 1.8or higher is recommended. DNA should be sterile and free of any contaminants such as endotoxins.
Choice of promoter
  High gene expression depends on both the promoter unter which the gene of interest is expressed and the targeted tissue/organ. Cytomegalovirus (CMV) promoter is best known for high gene expression in a wide variety of cell. Some researchers prefer LTR, simian virus (SV40)or Rous sarcoma virus (RSV) promoters.
Transfection Reagent/DNA Ratio:
The volume of transfection reagent used depends on the amount of DNA, transgene and animals to be transfected. The ratios presented in the protocols below are starting amounts and can be further optimized for best results.