T-Pro Biotechnology


for your life science lab

Chemiluminescent Substrates

T-Pro LumiNano Chemiluminescent Substrate Kit

T-Pro LumiFast Chemiluminescent Substrate Kit

T-Pro LumiFast Plus Chemiluminescent Substrate Kit

T-Pro LumiLong Chemiluminescent Substrate Kit

T-Pro LumiLong Plus Chemiluminescent Substrate Kit

 

The principle of T-Pro LumiNano, LumiFast, T-Pro LumiLong and Plus Chemiluminescent Substrate Kit is based on chemiluminescence and is very convenient to detect the Horseradish peroxidase (HRP) activity in many assays such as Western blottingSouthern and Northern. HRP catalyze the chemiluminescent oxidation of cyclic diacylhydrazides such as luminol by hydrogen peroxide (H2O2). T-Pro LumiNano,  LumiFast, T-Pro LumiLong and Plus Chemiluminescent Substrate Kit can enhance the luminol-dependent chemiluminescent and can be wildly use to detect the present of HRP-conjugated antibodies or streptavidin which binding to antigen or nucleotide sequence respectively.

Procedural

1

Mix the solution A 11 with solution B in T-Pro Chemiluminescent Substrate Kit and incubate the mixture for 1 min in room temperature.

2

Add the sufficient mixture solution to cover the membrane (0.1ml/cm2). Incubate the membrane for 1 min in room temperature.

3

Discard the excess mixture in membrane and wrap the membrane in saran wrap. Carefully and gently remove the air bubbles from the membrane.

4

Place the membrane in the film cassette and keep the protein side up. Turn off the lights and use safety light. Then place a sheet of film on the membrane and close the cassette and expose for 10-90 seconds.

5

Open cassette and transfer the exposed film to developing machine. Then place a new film on the membrane and expose again.

6

The exposure time of second film can be adjusted by the intensity of first film.

7

If the intensity was too high, please wait up to 10 minutes before re-exposing.

 

 


 

  • Pages: 1
  • No.JT96-K000S

    Chemiluminescent Substrates

    T-Pro LumiNano Chemiluminescent Substrate Kit (S)

    100ml x 2 ( reagent A 100ml + reagent B100ml )

     

    Signal Duration: 1 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/100 – 1/5,000

    Secondary: 1/1,000 – 1/20,000

    Lower Detection Limit: Low-Nanogram (10-9)

    High-Picomole (10-11)

    -
  • NoJT96-K000M

    Chemiluminescent Substrates

    T-Pro LumiNano Chemiluminescent Substrate Kit (M)

    250ml x 2 ( reagent A 100ml + reagent B100ml )

     

    Signal Duration: 1 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/100 – 1/5,000

    Secondary: 1/1,000 – 1/20,000

    Lower Detection Limit: Low-Nanogram (10-9)

    High-Picomole (10-11)

    -
  • NO.JT96-K001S

    Chemiluminescent Substrates

    T-Pro LumiFast Chemiluminescent Substrate Kit (S)

    100ml x 2 ( reagent A 100ml + reagent B100ml )

     

    Signal Duration: 6-8 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/1,000 – 1/5,000

    Secondary: 1/20,000 – 1/100,000

    Lower Detection Limit: Low-Picomole (10-11)

    High-Picogram (10-14)

    -
  • NO.JT96-K001M

    Chemiluminescent Substrates

    T-Pro LumiFast Chemiluminescent Substrate Kit (M)

    250ml x 2 ( reagent A 250ml + reagent B250ml )

     

    Signal Duration: 6-8 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/1,000 – 1/5,000

    Secondary: 1/20,000 – 1/100,000

    Lower Detection Limit: Low-Picomole (10-11)

    High-Picogram (10-14)

    -
  • NO.JT96-K002S

    Chemiluminescent Substrates

    T-Pro LumiFast Plus Chemiluminescent Substrate Kit (S)

    100ml x 2 ( reagent A 100ml + reagent B100ml )

     

    Signal Duration: 6-8 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/1,000 – 1/5,000

    Secondary: 1/20,000 – 1/100,000

    Lower Detection Limit: Low-Picogram (10-12)

    High-Femtogram (10-15)

    -
  • NO.JT96-K002M

    Chemiluminescent Substrates

    T-Pro LumiFast Plus Chemiluminescent Substrate Kit (M)

    250ml x 2 ( reagent A 250ml + reagent B250ml )

     

    Signal Duration: 6-8 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/1,000 – 1/5,000

    Secondary: 1/20,000 – 1/100,000

    Lower Detection Limit: Low-Picogram (10-12)

    High-Femtogram (10-15)

    -
  • No.JT96-K003S

    Chemiluminescent Substrates

    T-Pro LumiLong Chemiluminescent Substrate Kit (S)

    100ml x 2 ( reagent A 100ml + reagent B100ml )

     

    Signal Duration: 12 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/1,000 – 1/50,000

    Secondary: 1/50,000 – 1/250,000

    Lower Detection Limit: Low-Picogram (10-12)

    High-attomole (10-17)

    -
  • No.JT96-K003M

    Chemiluminescent Substrates

    T-Pro LumiLong Chemiluminescent Substrate Kit (M)

    250ml x 2 ( reagent A 250ml + reagent B 250ml ) 

     

    Signal Duration: 12 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/1,000 – 1/50,000

    Secondary: 1/50,000 – 1/250,000

    Lower Detection Limit: Low-Picogram (10-12)

    High-attomole (10-17)

    -
  • No.JT96-K004S

    Chemiluminescent Substrates

    T-Pro LumiLong Plus Chemiluminescent Substrate Kit (S)

    100ml x 2 ( reagent A 100ml + reagent B 100ml )

     

    Signal Duration: 12 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/1,000 – 1/50,000

    Secondary: 1/50,000 – 1/250,000

    Lower Detection Limit: Low-Picogram (10-14)

    High-Zeotomole (10-19)

    -
  • No.JT96-K004M

    Chemiluminescent Substrates

    T-Pro LumiLong Plus Chemiluminescent Substrate Kit (M)

    250ml x 2 ( reagent A 250ml + reagent B 250ml )

     

    Signal Duration: 12 hours

    Detection Method: X-ray film or imaging acquisition system

    Suggested Antibody Dilution: Primary: 1/1,000 – 1/50,000

    Secondary: 1/50,000 – 1/250,000

    Lower Detection Limit: Low-Picogram (10-14)

    High-Zeotomole (10-19)

    -
  • Pages: 1